Potential neuroprotective of trans-resveratrol a promising agent tempeh and soybean seed coats-derived against beta-amyloid neurotoxicity on primary culture of nerve cells induced by 2-methoxyethanol

Abstract Resveratrol, a natural polyphenol found in tempeh, has not been investigated especially vitro as neuroprotective agent against 2-methoxyethanol (2-ME)-induced beta-amyloid cytotoxicity. Beta amyloid peptides (Aβ) could initiate neurotoxic events and neuron-inflammatory response via microglial activation. However, it remains unknown whether the effect of and/or associated with potential 2-ME to induce effects on primary culture nerve cells induced by 2-ME. This study trans-resveratrol promising tempeh soybean seed coats-derived cytotoxicity 2-methoxyethanol. Biotium MTT assays were used analyze neurons, which isolated from cerebral cortex fetal mice at gestation day 19 (GD-19). A standard solution was dosed 10 μL. The cultured randomly divided into following groups: (1) group + resveratrol standard, (2) (3) coats, (4) control group, without addition either or resveratrol. Exposure cortical neuron monoclonal antibody pre-incubated for 24 h µL 4.2 µg/mL 7.5 mmol/l 2-methoxy-ethanol additions. Here, we report that (standard tempeh) cell concentrations 1.4, 2.8 showed majority neurons grew well. In contrast, after exposure Beta-amyloid, glial activated. These findings demonstrate role neuroprotective-neurorescuing action.